FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923045
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.2263161601005368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.20085694630272632No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC15980.17312265382511144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.1709559122252978No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA15240.1651057099058009No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15040.16293896830598725No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA13250.143546630987655No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC12980.14062152982790654No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG12880.1395381590279997No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG12880.1395381590279997No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA11360.1230709228694159No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG10560.11440395647016126No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT10020.10855375415066437No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT10010.1084454170706737No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA9710.10519530467095321No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC9610.10411193387104638No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT9280.10053681023135383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG2600.0107.538551
GGTATCA13700.072.516281
GTATCAA29950.050.6504141
GTGCACA505.3367356E-447.5915269
ACGTGGA558.535022E-443.2650221
ACTGTGC2400.042.1383328
GTACACT1104.7053072E-737.8568954
ATCAACG49650.037.2632562
AAATTGG1304.2078682E-836.6088681
CAACGCA51200.036.018984
TCAACGC52800.035.0401653
CTGTGCG1209.2987466E-734.7021569
CATCTAG1555.125912E-934.5422361
TTCTAAG700.002791137433.993952
AACGCAG54650.033.9628455
TATCAAC52050.033.9449651
AGTGCGG902.3951303E-433.0496758
ATCTAGG1301.7380007E-632.032762
TTCTCTG3900.032.0327577
ACGCAGA58200.031.7890156
Produced by FastQC (version 0.11.5)