FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004912486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48802795
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3155960.6466760766468396TruSeq Adapter, Index 2 (97% over 37bp)
CACCGTTCGGCCTCGGGCACCTGAGGGACAACCCGGAGCGCTCCAGGAGC1618930.3317289511799478No Hit
GGGGTCTTTAAACCTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGG1571380.32198565676412594No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT1232610.2525695505759455TruSeq Adapter, Index 2 (97% over 37bp)
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC915390.1875691750851565No Hit
GACCGGAGGAGGGGGCGCCGGGGGCGGGAACGACACACCACCGTTCGGCC908760.18621064633695672No Hit
GGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGC818260.16766662647088143No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT781280.1600891916128984No Hit
GGGTCTTTAAACCTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGGG688310.1410390531935722No Hit
GCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAACCCG684730.1403054886508037No Hit
GGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGG680710.13948176533741563No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC602460.12344784760790853No Hit
GGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAG570240.11684576672299199No Hit
GGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCG542240.1111083904108361No Hit
GCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGG514580.10544068223961353No Hit
CCACCGTTCGGCCTCGGGCACCTGAGGGACAACCCGGAGCGCTCCAGGAG504220.10331785300411586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)