FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004912530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47187133
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6156721.3047455118750275TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT2458060.5209174289101226TruSeq Adapter, Index 2 (97% over 37bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1445990.30643735019883495No Hit
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC1394430.29551064269999194No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1334510.28281226579288044No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1231830.26105209655352446No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1178080.2496612794848121No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1006120.21321914175205345No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT933180.1977615380870883No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG904620.1917090406827641No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA814290.17256611034198666No Hit
GGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGG728590.15440437968545365No Hit
GCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACG676350.14333356510555537No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG665200.14097063282060388No Hit
GGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGC641000.13584211611245803No Hit
GCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGCC637750.13515336903388472No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA627180.13291335161218631No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG588360.12468653266135071No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG571620.12113895540125313No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC570370.12087405267872495No Hit
GGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGC551250.11682210063493367No Hit
CCCCCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCC548690.11627957985919593No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC534230.11321518516498978No Hit
GCCGGTGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGG528310.11196060587109626No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT524470.11114682470748964No Hit
GGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGA511580.1084151478327789No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG509010.10787050783526095No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT500510.10606916932206921No Hit
GGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAG484140.10260000326784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)