FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8438948
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT88250.10457464603407912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG56050.055.093353145
TCTCGTT54200.042.663513145
ACCGCTA31950.041.523782
CGCTAAG32800.041.327164
TGCCGTG50350.037.57563145
CCGCTAA38000.037.009373
ATTATCG33100.036.572422145
CCGGTAA9900.035.151041
AGAGTCG41350.033.6617979
CGGTAAT11650.031.1143532
CGTCTTG83550.029.411587145
CATTATG116450.028.758596145
GTATCGA23450.027.2024234
CGTATCG24550.026.2804033
TATCGAA25400.024.8286655
CCCGTAT29400.024.659531
CCGTATC31350.022.8936122
TCGTACG30800.022.40809810-14
ATCGAAG29800.022.1356856
CACCGCG97450.020.75332145
Produced by FastQC (version 0.11.5)