FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8438948
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT25200.065.58563145
TCGTGTT85200.048.07101145
TGTGCCG138300.039.73026145
CCGTATA23200.036.8696145
TCTCGGG92500.032.443897145
ATGTGTT119900.031.377806145
AGAGCGG100600.029.183102145
TCAATCG22950.024.953513
TCGCATA7150.024.3321027
TCGCCGG67350.023.355896145
GTAGATG124950.022.683752145
CGGTAAT8500.022.1780851
CAATCGC25500.022.1736164
CGGTGGG161850.021.856544145
GTAATCG7850.021.2395553
GCCGTAA11650.020.533474145
GCGTCGG111500.020.088987145
TACCGTA26000.020.0757689
CGTACCG26150.019.4044238
CTACCGT27550.019.2077128
Produced by FastQC (version 0.11.5)