FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8288587
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT87170.10516870969683977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATCG27200.047.97472145
ATGCCGG45900.047.856266145
TCTCGTT46100.035.38266145
CGCTAAG31200.035.3177154
TGCCGTG41500.034.58804145
ACCGCTA31450.033.8870962
CCGCTAA36950.030.2150273
CCGGTAA10150.030.0025121
CGTCTTG69850.029.475475145
AGAGTCG39450.029.0367329
CGTATCG22300.026.6578883
CGGTAAT10950.026.4842
GTATCGA23300.025.2018684
CATTATG108100.024.880384145
CCCGTAT27550.024.7389131
GAGTATT44150.024.6319189
TATCGAA25600.023.2208185
CCGTATC28700.022.9877872
TCGTACG29450.022.15663510-14
TGAGTAT51900.021.7903148
Produced by FastQC (version 0.11.5)