FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8288587
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT21800.064.51307145
TCGTGTT71450.044.338573145
TGTGCCG111050.042.889336145
CCGTATA20550.034.57138145
TCTCGGG84000.032.277122145
ATGTGTT113350.028.780178145
TACCGTA24100.026.4700689
CTACCGT24600.026.2267388
TCGCCGG54150.025.034887145
AGAGCGG93700.024.989952145
CAATCGC23050.024.5308764
TCAATCG22500.024.4898413
TCGTCCG23700.023.2591112
TCGCATA5650.023.0947597
ACTACCG27800.022.686317
GTAGATG115800.022.662222145
CGTAACG3604.8676156E-922.15038
GTAATCG6700.021.6426333
GTAGATA85050.019.604511145
TACGGTC20100.019.1252772
Produced by FastQC (version 0.11.5)