FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8725740
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT94040.10777309431635597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG53300.053.592724145
ATTATCG32450.044.460587145
CGCTAAG33500.039.1737824
ACCGCTA31950.039.0332072
TGCCGTG49750.038.1808145
CCGGTAA11050.036.090221
CCGCTAA39250.033.619673
TCTCGTT50500.032.87615145
AGAGTCG40850.032.3008279
CGTCTTG86150.030.800846145
CGGTAAT12300.030.6531332
CATTATG113950.027.612915145
GTCGTGC32200.023.4174274
TCGTACG32400.021.39363310-14
GTATCGA24300.021.1842844
CGTATCG24400.021.0974643
CGTATGG17550.020.655214145
GAGTATT48450.020.649989
GTTGTCG37650.020.6066741
CCCGTAT28050.020.4212971
Produced by FastQC (version 0.11.5)