FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8672228
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT91160.10511716251002626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG57050.051.975796145
ATTATCG31750.051.149067145
CCGGTAA11700.044.6218641
TCTCGTT55650.041.818966145
TGCCGTG51300.040.70133145
ACCGCTA31950.040.6198352
CGGTAAT12400.038.5903052
CGCTAAG34800.038.541934
CCGCTAA38700.035.0325363
AGAGTCG42050.032.7586569
CGTCTTG83250.028.999697145
CATTATG113800.027.20314145
GTATCGA22450.024.2206554
CGTATCG22750.023.5825793
TATCGAA24050.022.6093035
GTCGACA9750.022.310931
TCGTACG33100.022.25359510-14
CCCGTAT26900.020.7558021
GAGTATT45600.020.509899
CACCGCG103700.020.274618145
Produced by FastQC (version 0.11.5)