FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8672228
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT24950.068.28656145
TCGTGTT87650.043.094685145
TGTGCCG129850.038.97188145
CCGTATA21150.034.96453145
ATGTGTT124000.032.274185145
TCTCGGG96150.031.292244145
TCAATCG25050.029.532533
CAATCGC27900.027.5551244
TCGCATA6950.026.078537
TCGCCGG67000.024.455217145
TACCGTA24600.023.871679
AGAGCGG98850.023.323214145
GATCAAT39450.022.6162931
TACGGTC22500.022.5645282
CTACCGT26150.022.4567158
TCGTCCG24500.021.9065443
ACTACCG29600.021.0636987
CGGTGGG162600.020.777979145
GTAGATA84400.020.358408145
CTACGGT25750.019.7189661
Produced by FastQC (version 0.11.5)