FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149839
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA48160.418841246470158No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA46740.40649169144549807No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC38390.33387282915260313No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC32730.2846485464486767No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC32640.28386582817246586No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC29420.255861907623589No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC27750.24133813516501007No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT25740.2238574269963012No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA23850.2074203431958735No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC23430.20376765790688955No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC22360.19446200728971622No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC21510.1870696680143916No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT20560.17880764176549935No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG20410.17750311130514795No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20140.1751549564765154No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG19080.16593627455669882No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA18560.16141390229414726No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT16450.14306350715187083No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT16200.1408892897179518No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA16000.1391499157708166No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC15790.13732357312632462No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA15760.13706266703425438No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA15180.13201848258756224No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG15030.13071395212721085No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC15000.13045304603514057No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG14730.12810489120650806No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG14690.127757016417081No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA14130.12288676936510243No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA13450.11697289794484272No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT13000.11305930656378849No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA12680.11027630824837216No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA12290.10688452905145851No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA12030.10462334292018274No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC11940.10384062464397188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.067.968481
GTATCAA30350.052.992841
TTCTCTG9250.045.0568057
TCTCTGC10100.041.2523278
ATCAACG40000.040.2083173
CAATACG758.133821E-539.711924
CAACGCA41200.039.3263665
TATCAAC41550.038.99512
TATTCTC11600.038.5137145
GTACTGG6550.038.1961981
ACGCAGA42850.037.932897
AACGCAG43950.037.6723256
CGCAGAG43150.037.5197458
GTTCTAA1751.0913936E-1137.442671
GGTTCAC5900.037.349626
GCAGAGT43650.036.8220569
CTACGTC1304.1607564E-836.6571543
ATTCTCT11900.036.034796
TCAACGC46250.035.1611484
TCAAGAC2101.8189894E-1234.0387883
Produced by FastQC (version 0.11.5)