FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973249
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC25390.2608787679206452No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG24110.24772694346462212No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC17580.18063208901319192No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG17470.1795018540990024No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC17330.1780633732991249No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC15650.16080160370059463No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT15410.15833563661509029No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA13970.14353983410206433No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT12120.12453133781796846No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG10960.11261249690469757No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC10340.10624208193381139No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG9970.10244038267699222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT4050.089.306095
TGCGACA4050.089.306094
CTGCGAC5450.066.365083
GCTGCGA6550.055.2198032
TAGCCGC1351.03791535E-842.9066121
TAGGCCC4750.042.680791
CACGAAT4300.040.3746645
CGCACTA7900.040.2853437
CGACATC10150.037.7705766
GGCTGCG9600.037.710891
TAGCCCC4500.037.006951
TCACGAA4350.036.58474
CCCGCCA11350.035.7240031
GTCCGCA8600.035.327834
TCCGCAC8650.035.1236275
AACCCGC1656.0863385E-835.1054081
TGTCCGC9250.033.627373
CATCACG4750.033.503892
ACATCTG11650.032.2848638
CATCTGT11950.032.079649
Produced by FastQC (version 0.11.5)