FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973249
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA19780.20323678729698155No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG19430.19964058529728773No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA17320.1779606246705622No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG13070.1342924575314231No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC12330.12668905901778477No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG12240.12576432136072063No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG11940.12268186250384022No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG11560.11877741461845837No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA10530.10819430587650232No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG10370.10655032781949944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT1450.069.955792
GTAGCAA23450.067.988089
ATACCGG3400.066.074799
AATACCG3500.064.1869358
CGACCTA1250.063.7531431
AGTAGCA25700.062.0358248
TAGTAGC27100.058.010867
CCGCTAT1154.0017767E-1156.700373
CCCTCGG21550.055.774284145
GGTGTTA4700.050.8721052
GTTAGTA4800.049.80975
GCTAGTA3200.049.80975
GCCCTAT4150.048.8797341
GATAGTA4050.048.300325
CGACACG450.0096651148.3003165
TCCGTTA901.3297849E-648.3003163
TTCCGAC4850.047.814688
GGTAGTA14850.046.348795
CCTATTC12750.046.027364
GACCTAT2050.045.9465682
Produced by FastQC (version 0.11.5)