FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891338
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC17470.19599747794888134No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC14680.16469622073781215No Hit
GGCAGTATGATGGCCTAGATGCAGAGAAAACAGCTCCTTGGTGAATTGAT9570.10736667796054919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT2150.0101.090885
TGCGACA2350.095.570314
CTGCGAC3050.073.636143
GCTGCGA3050.073.6278842
CGACATC3100.070.111426
GGCTGCG3700.062.682751
CTAGGCG6300.060.9418687
TAGGCGG7400.051.882948
GCTAGGC8600.044.648476
GGCTAGG10200.040.4859055
GGTGTGC6800.036.220177
TAGCCGA800.002044742536.220172
AGGCGGG11550.034.4953969
TGGCTAG12450.033.751094
GCGCGGT2009.0112735E-932.6018076
GCACGAC900.003633678432.211971
TTAGGCG900.003642677332.1957052
GGCTGGC12650.031.511711
CTGGCTA14500.028.4797423
GCTGGCT14150.028.157022
Produced by FastQC (version 0.11.5)