FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004912610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990372
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT187431.8925211940563749No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG155061.5656743122786185No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC94890.9581248258230242No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG81210.8199949110031381No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC81200.8198939388431822No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC79430.8020218665309602No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA71900.7259898300840493No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT70990.716801363528048No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC67060.6771193046653177No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG57680.5824074186265362No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT49310.4978937207433166No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC47210.47668956715254474No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG42860.43276667757165993No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG42000.424083071815439No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG40440.40833141486229413No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT39210.3959118391876992No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG38770.3914690641496327No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG37230.3759193515163999No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA36980.37339504751749847No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG35200.35542200304532034No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT35180.35522005872540824No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG34150.34481992624993435No Hit
GACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGC33930.3425985387309011No Hit
CGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCG31960.3227070232195579No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG30700.3099845310650947No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC30310.3060466168268085No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC29120.29403092979203777No Hit
TCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGA28220.2849434353959926No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG27980.28252010355704726No Hit
CCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCAT26890.27151413812183706No Hit
GGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCGGGCGCT26580.26838400116319927No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA25060.25303623284987864No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC23690.2392030469358988No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT21930.22143194678363282No Hit
CCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACT21650.21860472630486324No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC20170.2036608466313668No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG20050.20244918071189413No Hit
ATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGA19690.19881418295347608No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT19480.19669376759439888No Hit
GCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGT19390.19578501815479435No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG19360.1954821016749262No Hit
CCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTC18810.18992863287734307No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA18390.18568780215918868No Hit
CATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGC17970.18144697144103428No Hit
GCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG17900.18074016632134188No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA17880.18053822200142977No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT17800.17973044472178132No Hit
CCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT17290.17458086456402241No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG17070.17235947704498916No Hit
CGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCAT16580.16741184120714236No Hit
CCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGT16540.16700795256731812No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT16380.16539239800802125No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT16070.16226226104938346No Hit
TTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGA16020.16175740024960317No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC15840.15993990137039416No Hit
GGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGC15620.1577185138513609No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC14530.1467125484161507No Hit
CGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTC14470.14610671545641435No Hit
CCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTT14340.1447940773769856No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT14250.14388532793738112No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC14070.14206782905817208No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA13650.1378269983400177No Hit
CCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT13480.13611047162076473No Hit
CGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTAGCG12840.12964825338357708No Hit
AGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGAT12740.12863853178401652No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC12680.12803269882428017No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC12670.12793172666432412No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC12630.12752783802449988No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG12450.12571033914529087No Hit
CATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCAC12400.12520547834551057No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT12020.12136853626718042No Hit
CACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGCG11870.11985395386783955No Hit
CCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATTCAGAG11860.11975298170788351No Hit
TACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTA11660.11773353850876238No Hit
ATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTG11420.115310206669817No Hit
TGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGA11400.1151082623499049No Hit
CCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAAT11340.11450242939016854No Hit
TCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTG11300.11409854075034431No Hit
AGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGA11280.11389659643043219No Hit
CGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC11220.11329076347069587No Hit
TTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCT11160.11268493051095953No Hit
GGCTTGCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTT11150.11258395835100346No Hit
CCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTA10950.11056451515188233No Hit
GAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACA10630.1073334060332885No Hit
ACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAG10540.106424656593684No Hit
CGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGACC10290.10390035259478256No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA10210.1030925753151341No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG10180.10278965883526595No Hit
CACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTC10060.10157799291579325No Hit
CAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG10000.10097215995605692No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA9960.1005682713162327No Hit
CCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACG9950.10046729915627663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)