FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004912611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990372
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG154151.5564858457226174No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA138961.403109134749367No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA107691.087369190566777No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG82050.828476572439447No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG80900.8168647740445005No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG80350.8113113052469173No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA71200.7189217788871253No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG60350.6093669853348035No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC58210.5877589431042074No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG55740.5628188195950613No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC45570.46013013291975136No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG39050.3942962846284022No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG39030.3940943403084901No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG34240.34572867568953886No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG33160.33482368241428473No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG32220.3253322993784154No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG31300.3160428606624582No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA31230.3153360555427657No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC28850.2913046814732242No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC28250.2852463518758608No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC27450.27716857907937625No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC26020.26272956020566013No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA25650.258993590287286No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC25350.25596442548860426No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT24770.250108040211153No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT24480.24717984757242734No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC24110.2434438776540532No Hit
CAGCCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG23150.23375055029827177No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA22670.22890388662038103No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG22520.22738930422104017No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA22370.22587472182169932No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA22100.22314847350288577No Hit
GGCGCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC22060.22274458486306156No Hit
GGCATGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC22020.22234069622323735No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG20880.21082986998824685No Hit
GCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGA20690.20891139894908176No Hit
GGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTG20520.20719487222982882No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA19880.20073265399264115No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC19830.20022779319286088No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA18910.19093835447690363No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA18850.19033252151716729No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG18370.18548585783927657No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG18050.18225474872068273No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC18040.18215377656072668No Hit
CGACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG18030.18205280440077062No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC17710.17882169528217678No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA17430.1759944748034072No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC17400.17569155832353903No Hit
CAACCCATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG17030.17195558840516495No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC16720.16882545144652716No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG16650.16811864632683476No Hit
GCCCTGTCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG16600.16761378552705447No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC16090.1624642053692956No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA15730.15882920761087754No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15670.1582233746511412No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT15640.157920458171273No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA15510.15660782009184426No Hit
GGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTAC15390.1553961541723716No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA15050.15196310073386565No Hit
CTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACG14910.15054949049448085No Hit
GGCCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG14780.1492368524150521No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA14390.1452989381767659No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA14030.14166394041834784No Hit
GGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTT13840.13974546937918278No Hit
GGCCCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC13790.1392406085794025No Hit
CGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCT13720.1385338034597101No Hit
GTCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA12980.13106186362296188No Hit
CCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCAC12840.12964825338357708No Hit
GGGTGTAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC12640.12762881018445596No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT12480.12601325562515903No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA12470.12591228346520297No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG12270.12389284026608184No Hit
GCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCC12210.12328700730634548No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT12090.12207534138687282No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA12070.12187339706696071No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA12040.12157048058709252No Hit
AGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCG11960.12076270330744407No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA11950.120661731147488No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA11300.11409854075034431No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC11190.1129878469908277No Hit
GCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAG11130.11238201403109135No Hit
CACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG11060.11167520891139895No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG10870.10975673787223386No Hit
GGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAG10860.10965576571227781No Hit
TAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG10620.10723243387333245No Hit
GGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTC10490.10591979579390372No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA10470.10571785147399158No Hit
TGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACGC9980.1007702156361448No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT9960.1005682713162327No Hit
GCCCAATGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA9920.10016438267640847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)