FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768699
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT275730.5782080185811685No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG234870.49252427129495907No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT203410.4265523993021996No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT177470.3721560115243172No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT122520.25692542137803204No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA120200.25206036279496774No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA77100.16167931756648932No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT72630.15230569176205083No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA66880.14024789570488722No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC62410.13087426990044873No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC57810.12122803305471787No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC57530.12064087081193424No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC57360.12028437945024419No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA57070.11967624712736116No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT56400.11827125176070036No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA53870.11296582149554837No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT52890.1109107536458057No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52770.11065911268461272No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC51840.10870889523536713No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT51470.10793300227168877No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51370.10772330147069463No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG47860.10036280335579999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60800.052.1340681
GTATCAA124600.051.9786571
ATCAACG152400.041.558693
CAACGCA153650.041.3369265
AACGCAG153600.041.3364836
TCAACGC155900.040.7021184
ACGCAGA156900.040.4969567
CGCAGAG156900.040.4799548
TCTATAC41950.040.0525973
ACCTTAG45900.039.980811
TATCAAC169650.038.983662
GCAGAGT164800.038.624689
TATACCA40200.037.4979935
GGGGTTA120350.036.672436
CCTTAGC48150.036.627642
AGGGGTT105800.036.2671745
TTAGCCA49450.036.1466874
GGGTTAG120450.035.991967
CCCTGGG14600.035.5042152
TACATGC13500.035.3077242
Produced by FastQC (version 0.11.5)