FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739186
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT67750.1811891679098071No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG56180.15024660447487767No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT48030.12845041674845808No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT47590.12727369004911765No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC46660.12478651770732989No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT45960.12291445250383372No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT43730.11695058764126737No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA38910.10406008152576525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29950.068.8242651
GTATCAA81850.058.5193751
ATCAACG95200.048.8738333
AACGCAG97050.047.92876
CAACGCA98050.047.331715
CGCAGAG98600.047.0896268
ACGCAGA98750.047.0338367
TCAACGC99100.046.9504474
AGGACCG5300.043.8380365
GCAGAGT108000.043.1639489
TCTATAC38450.043.0735633
TATCAAC111050.042.541912
TATACTG52500.042.326455
GACCGTT5700.041.78677
TCATACG1151.4118996E-841.4433145
ACTGTAC50800.040.42628
GTATATA83500.035.459471
TACTGTA56000.034.6643077
ATACATG38150.034.6390538
TATATAA76500.034.34312
Produced by FastQC (version 0.11.5)