FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391700
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC217950.9112764978885312No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA217360.908809633315215No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA201850.8439603629217711No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC155210.6489526278379396No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC148180.6195593092779195No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC132880.5555880754275201No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT123850.5178325040765983No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC110360.4614291090019651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94910.3968307061922482No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC91080.3808169920976711No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA88730.3709913450683614No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC87270.36488690053100303No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA86970.36363256261236776No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG86880.3632562612367772No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC86790.3628799598611866No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC86740.36267090354141407No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT79370.33185600200694065No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA77010.32198854371367647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.2835221808755279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.2823096542208471No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC67060.2803863360789397No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA63640.2660868838064975No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT63320.2647489233599532No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA62710.2621984362587281No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG59610.2492369444328302No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG55890.23368315424175273No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG55810.23334866413011662No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA55540.2322197600033449No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA52040.21758581761926665No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT49350.2063385876155036No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA47940.20044319939791783No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA46100.19274992683028808No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC44720.1869799724045658No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG43840.18330058117656894No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT42930.17949575615670862No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG42220.17652715641593844No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA41910.17523100723334867No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC41280.17259689760421457No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT41120.17192791738094243No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA39590.16553079399590248No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT39280.1642346448133127No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC38910.16268762804699585No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA38870.16252038299117783No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA38750.16201864782372372No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT38490.16093155496090647No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT37630.15733578626081865No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG36710.1534891499770038No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC36650.15323828239327675No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG36650.15323828239327675No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT36480.1525274909060501No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT36190.1513149642513693No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG35570.14872266588618974No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG33750.14111301584646904No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG33450.13985867792783377No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC33340.13939875402433416No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA33090.1383534724254714No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT32950.1377681147301083No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA32870.13743362461847222No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC32540.13605385290797342No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG32070.13408872350211148No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA31950.13358698833465735No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC31670.13241627294393107No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT30880.12911318309152486No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA30700.12836058034034367No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA29990.12539198059957352No Hit
TAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCT29830.12472300037630138No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT29310.12254881465066689No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT29200.12208889074716729No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT28830.12054187398085044No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA28430.11886942342267007No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT27780.11615169126562697No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA27410.11460467449931012No Hit
CTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGG27000.11289041267717524No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG26930.11259773382949366No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA26750.11184513107831251No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT26750.11184513107831251No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT26000.1087092862817243No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT25930.10841660743404274No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT25910.10833298490613372No Hit
TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA25860.10812392858636118No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA24370.10189405025713928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39550.081.069961
GTATCAA83500.062.264811
TATTCTC31400.051.7147455
ACCTAGT350.00780432550.9883461
TTCTCTG32200.050.0594067
TCTCTGC33700.047.831248
ATTCTCT36000.045.6024256
TATCAAC119700.043.879032
ATCAACG124550.041.9315833
TAGTACT7000.041.6374
CAACGCA126950.041.1379975
CTCTGCA40300.040.88349
AACGCAG136250.039.0285456
CTGTGCG7500.038.0673149
ACGCAGA138750.037.5957647
TCAACGC140000.037.4732974
CGCAGAG139650.037.396068
GCAGAGT144600.036.1159069
TACTGTG11500.035.688117
ACTGTGC9900.035.4477848
Produced by FastQC (version 0.11.5)