FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391700
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA216190.9039177154325376No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC197520.8258560856294686No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA192530.8049922649161684No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT145210.6071413638834302No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC144200.6029184262240248No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC137100.5732324288163232No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC134200.5611071622695154No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC106740.4462934314504327No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA104310.43613329430948694No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC90150.3769285445499017No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC87180.3645105991554125No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC84440.3530543128318769No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC84170.35192540870510514No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA82540.34511017268052013No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG76600.32027428189154156No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT73250.30626750846678097No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA70900.29644186143747125No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA66740.2790483756323954No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG64540.2698498975624033No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC63610.26596145001463395No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG62460.2611531546598654No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT57540.24058201279424676No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA57180.23907680729188444No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG55700.23288874022661704No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT54070.226073504202032No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC53980.22569720282644146No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA48150.2013212359409625No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG47770.19973240791069116No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA47080.19684743069783No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT47040.19668018564201195No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA45260.18923778065810928No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG45000.18815068779529204No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA44620.1865618597650207No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA43850.18334239244052347No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC43090.18016473637998076No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT42880.17928669983693607No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA40480.16925199648785383No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG38690.16176778023999666No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT38680.16172596897604216No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT38260.15996989588995275No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT37700.15762846510850023No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT37000.15470167663168458No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA36990.15465986536773005No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG35160.14700840406405485No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC34990.1462976125768282No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA34430.14395618179537567No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC34060.14240916502905882No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT33830.14144750595810512No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT33690.140862148262742No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG33480.13998411171969727No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA33330.13935694276037963No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC32400.13546849521261026No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG31730.13266714052765816No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG31590.13208178283229502No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC31370.1311619350252958No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG31310.13091106744156877No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT30430.12723167621357195No Hit
TAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCT29660.12401220888907472No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA29520.12342685119371159No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA29470.12321779487393904No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA29360.12275787097043944No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA29250.12229794706693983No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC29040.12141991052389514No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT28830.12054187398085044No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA28690.11995651628548731No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT27680.11573357862608186No Hit
CTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGG27580.11531546598653676No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC26840.11222143245390309No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA26810.11209599866203955No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT26400.11038173683990467No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG25910.10833298490613372No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA25700.10745494836308901No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT25470.10649328929213529No Hit
TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA25410.10624242170840824No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA25090.10490446126186395No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA25030.1046535936781369No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT24950.10431910356650083No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT24730.1033992557595016No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT24590.10281389806413847No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT23990.10030522222686791No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG23950.10013797717104989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.075.97491
GTATCAA51400.062.007931
TATTCTC34650.055.017815
TTCTCTG35850.052.8239447
TCTCTGC38500.049.3179058
ATTCTCT39600.048.579696
TATCAAC70500.045.1241152
GGGTAAA1850.045.0830041
ATCAACG69700.045.0437363
CAACGCA70450.044.4796455
CTCTGCA44600.042.7203949
ACGCAGA73600.042.1553577
CGCAGAG73950.041.6933028
GCAGAGT76050.040.5555889
AACGCAG78750.040.2354136
TCCTAGG801.1891306E-437.233732
TCAACGC85700.036.9817434
GGTCTAT2450.034.042271
AGTAGAC700.002777774434.029447
ACTGGTT7100.033.56283
Produced by FastQC (version 0.11.5)