FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004912651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966488
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA535591.35028771043805No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC444711.1211681467333319No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG317770.8011369251589819No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA316360.7975821431956934No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC314820.7936996153776339No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC311060.7842201968088648No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC302660.7630427723467208No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA258340.6513066470893143No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT257390.6489115812275242No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC246070.6203724806428256No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC231570.5838162122260296No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA216190.5450413564846281No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA214360.5404277032982326No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG207680.5235866086069088No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA191890.48377809286199785No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC179640.4528943488547047No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA166650.42014497459717515No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA154590.38974024376224004No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA150680.379882656899504No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT143820.36258776025541994No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA143420.3615793114715083No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC141770.3574194602378729No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG134400.33883879139430145No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA130470.32893078209236987No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC128210.3232330464632693No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC126660.31932530742561177No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT125600.31665291814824603No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT124160.3130225025261642No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG122310.30835842690057297No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT120980.3050053346940669No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG120670.3042237868865354No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG112850.2845086131610634No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA107430.27084413213906106No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA106360.2681465316420975No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT105670.26640695748985No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT104380.26315471016173503No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA97400.2455572788824774No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA96370.242960523263905No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT95940.24187644082120002No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA94960.23940574130061654No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA93410.23549800226295908No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG93100.2347164544554276No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG93000.23446434225944968No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT91150.22980026663385844No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT90880.22911956370471812No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC89710.22616985101177664No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC89590.22586731637660318No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG88940.22422858710274682No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA88630.2234470392952153No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA85930.21664001000381194No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA83560.21066495095913565No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG83200.20975734705361518No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC79980.20163933434312672No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC79180.1996224367753035No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA74750.1884538664934824No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT74280.18726893917238624No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT73920.1863613352668658No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA72910.183815002087489No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA72680.1832351440367398No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC71780.18096613427293867No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC70920.1787979693875287No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA68310.17221784107250546No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA68090.17166319424135407No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC66440.16750334300771866No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG63710.16062068005752192No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT62950.15870462736808985No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT58850.14836802733299584No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA58580.1476873244038555No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG58080.146426763423966No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG57700.14546873707924995No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC57460.144863667808903No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT57060.14385521902499138No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT56270.14186353267676594No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA54850.13828353949387973No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT54830.13823311705468416No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT52320.13190510093563879No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT51660.13024116044218462No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC50910.12835031897235036No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC50770.1279973618979813No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT50320.12686285701608072No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG49410.12456863603268181No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA48900.1232828638331945No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG48890.1232576526135967No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC47970.12093822041060001No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC47510.11977850430910164No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT47470.11967765943071049No Hit
TAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGT47110.11877005552519003No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA46540.11733301600811599No Hit
GATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATAT46500.11723217112972484No Hit
GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC45910.11574470917345521No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG44030.11100499988907064No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT43300.10916458085843193No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA42790.10787880865894464No Hit
CATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTA42130.10621486816549047No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT41890.1056097988951435No Hit
GTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGA41640.10497951840519874No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT41550.10475261742881864No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG41240.10397106962128716No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA41190.1038450135232982No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT40200.10134910278311694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)