FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7701759
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG86600.042.5849233
TCAACGC86850.042.393744
CAACGCA87150.042.384535
AACGCAG91000.040.9069826
GTATCAA97350.040.2692031
CGCAGAG92950.039.514338
GGTATCA49750.039.0398061
ACGCAGA95250.038.7605137
TATCAAC120550.031.432072
GCAGAGT123100.030.0374139
AGTACGG110200.027.09373712-13
AGAGTAC118000.026.59205410-11
TAGGGGT25400.025.5667724
TACGGGT14600.024.87785714-15
ATAGGGG27100.023.9629523
GAGTACG112700.023.4551712-13
GTACCGC24400.023.4336666
AGTACCG25850.023.2779065
TACCGCA25700.023.1701957
ACCGCAA26350.021.913418
Produced by FastQC (version 0.11.5)