FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004912656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192855
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA596531.4227298582946464No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC470941.123196485449652No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC423601.0102901245094333No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA352790.8414075850464658No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG318720.7601503033136132No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC292820.698378551130435No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC290190.6921059755226451No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA258220.6158572142370772No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC214520.5116322887388187No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC208650.4976322815837896No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA202650.483322223162976No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA189160.4511484418135137No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT181870.4337617208322253No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT178410.42550958714288944No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA172530.4114857298904923No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC171840.4098400731720987No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC158980.37916884795682176No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC156150.37241927040167144No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC155580.37105981485169415No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA151750.3619252275597415No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC151030.3602080205492439No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT148890.3551040997124871No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA145860.34787752020997625No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA132840.3168246934368109No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA132360.3156798887631459No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA130340.31086216909480535No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA129030.3077378063395944No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG126050.3006304773239237No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC121690.29023183487146587No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG121570.2899456337030496No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA116320.2774243325848378No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT110400.26330507494296845No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT110140.2626849724113999No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT108340.2583919548851558No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG102750.24505975045643122No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA101210.24138683546175577No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG99590.23752311968813614No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA99080.236306764722367No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC98970.23604441365131873No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT91580.2184191916963501No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG90580.21603418195954785No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT90430.21567643049902752No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA88780.2117411644333038No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG86590.20651799310970687No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC83740.19972071535982044No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT83070.19812275883616293No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT82790.19745495610985833No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA82500.1967633032861857No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC82450.19664405279934558No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT79810.19034762709418762No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG77200.18412275168113373No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT76560.1825963454495803No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA75810.18080758814697862No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG73430.17513126497338924No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA72480.1728655057234271No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC72180.17215000280238643No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA72080.1719115018287062No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA71580.1707189969603051No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA70730.16869173868402318No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG67800.16170366015519258No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA67730.16153670947361642No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG67410.1607735063578397No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG66660.15898474905523802No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT65410.1560034868842352No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA65120.15531183406056256No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT64620.15411932919216143No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT64560.1539762286079533No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT64050.15275987364218416No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT62320.14863380679751625No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA62300.1485861066027802No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA61000.1454855939449373No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC60280.14376838693443966No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT59270.1413595271002694No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC57690.13759121171612185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57500.1371380598661294No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC57170.13635100665298466No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA56030.1336320955530301No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT55720.13289274253462138No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG54790.13067468347939531No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT54650.13034078211624298No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA53610.12786037198996864No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC53300.12712101897155997No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG52970.1263339657584152No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG51400.12258950047163567No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG51320.1223986996926915No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG50320.12001368995588925No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT49310.11760483012171898No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA48740.1162453745717417No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT48670.11607842389016554No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT48480.1156252720401731No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA48200.11495746931386847No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG48140.11481436872966035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47740.11386036483493943No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC47600.11352646347178713No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC47570.11345491317968305No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT47260.11271556016127438No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT47110.11235780870075401No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG46660.11128455431919301No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT46320.11047365100868024No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT45630.10882799429028668No Hit
ACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATAT44680.10656223504032455No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT44120.1052266295877153No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA43730.10429647579036241No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA43670.10415337520615427No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT42770.10200686644303225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)