FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004912659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023547
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA319541.0568382102213063No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA285560.9444536499680674No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC282900.9356560357752005No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC192140.635478793615578No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC191490.633329000673712No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC183780.6078291490094251No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC173130.5726056185003905No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT158320.5236234131634137No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC154640.5114522777386956No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA151770.5019601150569183No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC143940.47606337854182523No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG135710.4488436925240455No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC111380.36837528902312416No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA109470.36205820514779496No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA109040.3606360344324067No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC108480.3587839051286453No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC101150.33454085549191065No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT99320.3284883615171188No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA98500.325776315036611No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG97400.32213820390422243No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG95780.3167802584183411No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT88540.292834872419711No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT80010.2646229742749162No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC78260.25883507020066165No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA78070.2582066691868855No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG77980.257909005548781No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA77470.25622224493285534No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA76720.2537417146153177No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA72420.2395200074614352No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA67330.22268547503974637No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA66470.21984113360896984No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT66090.21858433158141746No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG65210.21567384267550663No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA62860.20790151434722198No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG61950.20489180422860964No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC61850.20456106685293796No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT60450.19993074359353435No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA57290.18947944252230906No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC54300.17959039498972565No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA52980.1752246616308594No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT52070.17221495151224703No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT51480.1702636009957841No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT50730.16778307067824647No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG50330.1664601211755597No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG49020.1621274615542606No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG48990.1620282403415591No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC48640.1608706595267082No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA48610.1607714383140067No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT47880.1583570554716034No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC46310.1531644786735579No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT45800.15147771805763233No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT45650.1509816119941248No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA44810.1482034180384826No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT44560.1473765745993034No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT43020.14228321901395943No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA42770.14145637557478022No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG42420.14029879475992932No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG41160.13613150382646605No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC40080.13255954016921184No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA39910.13199728663056998No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT39750.13146810682949528No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG39690.13126966440409227No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG39690.13126966440409227No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT39450.13047589470248025No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC39000.12898757651195764No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT38960.12885528156168896No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT38690.1279622906473754No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG38630.1277638482219724No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA38010.12571327649280795No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT37700.12468799062822572No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG36860.12190979667258356No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA36480.12065299464503115No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG36120.11946234009261307No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT35760.11827168554019502No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT35720.11813939058992634No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA34960.11562578653482151No Hit
TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA34800.11509660673374683No Hit
CTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGG34740.11489816430834382No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT34260.11331062490511973No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC34140.11291374005431369No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT33400.11046628347434322No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA33380.11040013599920888No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA33270.11003632488597002No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT32900.10881259659598479No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA32880.10874644912085045No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA32410.10719198345519351No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC31870.10540600162656641No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA31760.10504219051332755No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT31680.1047776006127902No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA31680.1047776006127902No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA31540.10431456828684985No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA30670.1014371531185062No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT30630.10130485816823752No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT30550.10104026826770016No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA30460.10074260462959563No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30370.10044494099149112No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC30330.10031264604122245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)