FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004912672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929745
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA137440.7122184537335244No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC135030.6997297570404379No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC134430.6966205379467235No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA118330.613189825598719No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG82150.42570391424773746No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC77490.4015556459532218No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC72050.3733653928368774No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT65580.3398376469429899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64840.3360029433940754No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA63880.33102819284413226No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC63340.3282298956597892No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA61840.3204568479255031No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC58970.30558441659390234No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC56770.29418394658361596No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC53720.27837874952390085No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT53480.27713506188641507No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC53450.2769796009317293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53200.2756840929760149No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA44440.2302894942077839No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT43180.22376013411098358No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA39600.20520846018515398No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC39330.20380931159298252No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA38470.1993527642253251No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA38380.19888638136126793No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA37810.1959326232222392No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA37150.1925124822191533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37050.19199427903686755No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC37010.19178699776395328No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA36140.1872786300780673No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA34580.17919466043440974No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG33910.17572269911309524No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT32130.16649868246840904No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA31920.16541045578560898No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG31760.16458133069395178No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA31750.16452951037572322No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT31470.16307854146532313No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC31410.1627676195559517No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT29760.15421726704823693No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT29670.1537508841841798No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC29040.1504862041357796No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA28910.14981253999880814No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG28850.14950161808943668No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT27560.1428167970379506No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT26820.13898209348903612No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG26580.13773840585155034No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA26020.1348364680307502No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT25780.1335927803932644No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA25690.1331263975292072No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC25600.13266001466515007No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG25570.13250455371046432No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG25470.1319863505281786No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT25420.13172724893703572No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA25370.13146814734589285No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC24940.12923987366206416No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT24750.12825528761572125No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA24500.1269597796600069No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA23980.12426512311212103No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT23740.12302143547463523No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG22790.11809850524292069No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT22670.11747666142417781No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT22470.11644025505960633No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG22150.11478200487629193No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG21940.11369377819349188No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT21890.11343467660234902No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT21830.11312375469297757No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG21760.11276101246537754No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA21660.1122428092830918No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC21280.11027363719040599No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA21150.10959997305343454No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG21030.10897812923469163No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG20890.10825264477949158No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA20770.10763080096074869No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC20350.10545434759514859No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG20270.10503978504931999No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT19850.10286333168371987No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA19780.10250058945611984No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG19340.10022049545406259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)