FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004912687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469999
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA159440.6455063342130908No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC156040.6317411464539054No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA134560.5447775484929346No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC124200.50283421167377No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT99310.4020649401072632No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC94590.3829556206298059No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC90900.3680163433264548No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC89630.36287464083993554No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA77290.3129151064433629No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG72210.292348296497286No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT71810.29072886264326425No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA67430.2729960619417255No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC65650.26578958129132846No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC60920.24663977596752062No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC58790.23801629069485453No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC58700.23765191807769964No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC56400.22834017341707424No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA51140.20704461823668757No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC48470.19623489726109203No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG48340.1957085812585349No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA45530.18433205843403175No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA44760.1812146482650398No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG43290.17526322885150966No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA41930.1697571537478355No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT40820.16526322480292502No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG40270.16303650325364505No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA40050.16214581463393304No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT39710.16076929585801453No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC39130.1584211167696829No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA36520.14785431087219064No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA36310.1470041080988292No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA36260.14680167886707646No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT35760.14477738654954922No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA35670.1444130139323943No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT35420.14340086777363067No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT34410.13931179729222562No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC34150.13825916528711144No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA33940.13740896251375No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT33830.13696361820389402No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG31520.1276113876969181No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA30830.12481786429873049No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT30470.12336037383011086No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG30350.12287454367390432No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG29690.12020247781476835No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA29500.11943324673410799No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA29160.11805672795818944No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT29080.11773284118738511No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT28770.1164777799505182No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT28590.1157490347162084No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG28060.11360328485962949No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG27700.11214579439100987No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG27510.11137656331034952No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT27470.11121461992494733No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT27350.11072878976874079No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA27170.11000004453443099No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG27160.10995955868808044No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC26140.10583000236032483No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG25600.10364376665739541No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT25340.10259113465228124No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA25070.10149801680081653No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA24730.10012149802489799No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)