FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004912716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49825909
Sequences flagged as poor quality0
Sequence length20-50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGAT1380060.2769763819060481No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAA1059220.21258417984908215No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTAT921290.18490179476705582No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC819710.1645148109590936No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATA784330.15741408751820263No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT706510.1417957071289959No Hit
TGGATAATGAATTATAATTAATATTATTAATTAATATTTATTATTAATA704040.1412999811001943No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTACGTCGTTGTATTTTAGTT665340.1335329376529789No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTAT617960.12402382864705991No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGT542020.10878276199637422No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTT519260.10421485737470439No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT503520.10105585830857597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)