FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67332201
Sequences flagged as poor quality0
Sequence length20-50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTAT1111570.16508742971286502No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC973260.14454599516210676No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTAT756920.11241575186291623No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT732450.10878153233101646No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTACGTCGTTGTATTTTAGTT727140.10799290520742075No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGT680360.10104526361762627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGC1050.085.62533644
ACTATGC254.1136962E-579.9169844
TCGAGGC386050.077.7071244
CCGAGGC3500.071.3544444
CGCGATC15500.064.77141644
CCGGACC4400.062.4351444
TTGAGGC196100.062.22500244
TCAAGGC900.061.04768844
TTAAGGC109300.054.4265344
TGTGCCC150.00624544453.94561442
TTCAGTT155750.050.44518744
CTTCAGT10100.044.1665143
CGCACTA254.532149E-543.85647231
TCGAAGC10500.043.76405744
TCAAGAC801.526405E-743.70459744
CGTTCAC1153.274181E-1143.4331444
TTGAGGT3457000.042.59965544
CGTTTAC68350.042.4577244
CGGACTA1500.041.5281331
CCCGGTG303.7837017E-641.498655
Produced by FastQC (version 0.11.5)