FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656455
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20520.12387900667389093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.071.744531
GTATCAA40100.057.3118821
ATCAACG50850.044.493283
TATCAAC52600.043.2393762
CAACGCA53900.042.3069535
TCAACGC54850.041.57424
AACGCAG57100.040.2402886
CGCAGAG57650.039.2285588
ACGCAGA57900.039.0603567
ATCGGTA650.001936644236.6250157
CGGTATG851.706347E-435.015559
GCAGAGT64850.034.6969839
AGAGTAC61700.030.4886410-11
AGTACGG66250.027.53856812-13
CAGAGTA67450.027.35998710-11
GAGTACG65300.027.02764912-13
TTATCGG900.00949973526.4617925
GACCGTT1651.10795445E-525.2490637
CGCTAAC851.3980927E-524.50385362-63
TCTGTCG4650.024.3174868
Produced by FastQC (version 0.11.5)