FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049004
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60330.5751169680954505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43400.413725781789202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.3106756504264998No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12580.11992327960617881No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11960.1140129112949045No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.10305013136270214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50400.054.658321
GGTATCA30550.052.195061
ATCAACG55600.050.5046843
TCAACGC58400.048.18284
CAACGCA59350.047.411555
CTCAACG1650.046.8775861
AACGCAG60800.046.2808466
TATCAAC63900.044.1308142
TCCGAGA1950.042.712783
CGCAGAG66800.042.1218768
GCAGAGT69600.040.4273159
ATACCGA905.1610277E-639.6599776
ACGCAGA74850.037.5917367
CAACGGT3200.037.181234
CCTAAGA5150.035.81122
GTGGTAT6150.035.795831
ACAACGG3350.035.5180933
CACAACG3300.034.2534332
CAGTACG700.002789226633.9991261
GACTCTC6700.033.738977
Produced by FastQC (version 0.11.5)