FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049004
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49120.46825369588676496No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12690.12097189333882426No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10810.10305013136270214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG1101.7644197E-1048.699628
ATCAACG28750.047.4533963
CAACGCA30400.044.6818245
TCAACGC30350.044.559144
AACGCAG31650.043.471466
GTATCAA33200.042.349041
CGCAGAG33100.040.820068
GCAGAGT36450.037.0808039
ACGCAGA37100.036.275857
TATCAAC40550.034.672952
GTCTCGC1953.8198777E-1133.6068421
GGTATCA24850.033.084331
TATACCG750.00388604331.7737455
AGACCTT4750.030.0942556
ATACCGA1004.4115778E-429.7807736
ACAACGG2701.8189894E-1228.684633
GACTCTC6400.027.914147
GTACTGG2803.6379788E-1227.6601771
AGAGTAC36400.027.3989710-11
CAGAGTA39000.026.94641110-11
Produced by FastQC (version 0.11.5)