FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353917
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297710.6837750926349767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177320.4072654577475869No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134060.3079066504942561No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT74690.17154667854256295No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC72350.16617220769252147No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT58500.1343617712510367No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG51630.11858287606309444No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT51120.11741151703167514No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA49350.11334621215792584No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC48300.11093459062265082No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA47810.10980916723952248No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA45960.10556011977260935No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT45340.10413611467558982No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC45020.10340114430293457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97500.083.81371
GTATCAA227100.067.4328841
TATCAAC306700.050.388612
ATCAACG310700.049.5866783
TCAACGC324500.047.7319034
CAACGCA325350.047.5119325
AACGCAG333200.046.499726
ACGCAGA357850.043.1782847
CGCAGAG364600.042.3462648
GCAGAGT380950.040.497579
TAACGCA3750.031.7356954
AGAGTAC377800.031.5122610-11
CAGAGTA385300.030.70584110-11
GTACTGG42850.029.3076271
GAGTACT204350.027.16448212-13
GACCGTT9650.026.5115787
ACTGGTT43950.025.5904053
GTACGGA7700.025.5077571
GAGTACG247150.025.45740912-13
AGTACGG245800.025.40358412-13
Produced by FastQC (version 0.11.5)