FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353917
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC75200.17271803757398224No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT73740.16936473524874268No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT64180.14740749536566727No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT54440.1250368346479733No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA52090.11963939597378637No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52020.11947862120476802No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG49710.11417305382716299No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA47860.10992400636024988No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA43850.10071390887791383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.071.711141
GTATCAA136900.062.2476121
TATCAAC182500.046.367932
ATCAACG181950.046.3444483
CAACGCA188000.044.7580155
TCAACGC189600.044.411724
AACGCAG191800.044.0683256
ACGCAGA196550.042.9720657
CGCAGAG201550.041.8030178
GCAGAGT211350.039.7275549
GTACTGG39600.032.933311
AGAGTAC208700.031.11155910-11
CAGAGTA214750.030.63691510-11
ACTGGTT41200.027.6072183
GGTTCAC45450.026.8476836
TACTGGT46600.025.686012
GCTACCG14800.025.337037
GAGTACG258100.024.9621412-13
AGTACGG256650.024.7321312-13
GTTCACT49450.024.5550447
Produced by FastQC (version 0.11.5)