FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841786
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142480.5013748396255031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100690.35431943151243617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97360.3426014485256807No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC31630.11130324380512818No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA30870.10862886930965246No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT28430.10004271961365141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.081.823151
GTATCAA144100.058.0210841
TTACGGG7750.052.213241
TATCAAC210900.042.7451252
ATCAACG214250.041.9934463
CAACGCA220850.040.7079665
TCAACGC222350.040.596764
AACGCAG225500.039.9740756
ACGCAGA237850.037.8721357
CGCAGAG240350.037.4782078
GCAGAGT250750.035.876329
TAGTACG5700.035.495851
TGTATCA17150.034.351411
AGAGTAC248000.031.5959310-11
TTATCAA22600.030.017181
CAGAGTA251450.030.01483210-11
AGTACGG171000.026.35624912-13
GAGTACT122450.026.31096312-13
GAGTACG171900.025.11068712-13
TACTGGT21400.023.078892
Produced by FastQC (version 0.11.5)