FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904445
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37020.12745980729536968No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA36840.12684006755163207No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT35300.12153784974409912No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT34590.11909332075491187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33640.11582247210740779No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30650.10552790636421071No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC29320.10094871825770499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.058.9019131
GTATCAA98450.056.420931
TATCAAC130850.042.3594472
ATCAACG131050.042.203943
AACGCAG134400.041.4056366
TCAACGC135600.041.0512354
CAACGCA135950.040.9017565
ACGCAGA140800.039.3531237
CGCAGAG145900.037.967718
GCAGAGT147750.037.2922369
GTACGGA8000.034.976971
AGAGTAC146800.028.96233410-11
CAGAGTA155950.028.04525210-11
ACGGGTT9550.024.9362375
TACGGAA6300.024.570112
GAGTACG184200.023.292812-13
TACGGGT29500.023.2086642
CTACACT11300.023.1818754
AGTACGG182350.022.76221512-13
GAAAAAA136950.022.5186331
Produced by FastQC (version 0.11.5)