FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3093654
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45910.14840056451044623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.068.336761
GTATCAA85600.058.9108851
ATCAACG109400.044.897543
AACGCAG113950.043.7698556
TCAACGC113400.043.4188544
CAACGCA113850.043.2995345
TATCAAC114550.043.2948072
ACGCAGA117750.042.001477
CGCAGAG121650.040.792478
GCAGAGT130700.037.5612079
AGAGTAC122400.031.50702710-11
CAGAGTA126950.030.91689710-11
GTACGGA4050.030.8709641
AGTACGG143600.026.19395312-13
GAGTACG150150.025.5467712-13
GTACTGG17800.021.406511
ACGGGAT13450.020.57871416-17
GACCGTT5500.020.559637
GTACGGG189050.020.16549714-15
TACGGGC35800.019.61848414-15
Produced by FastQC (version 0.11.5)