FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415684
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG64940.2688265518172079No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT61630.2551244285262476No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA50010.20702211050783134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36900.15275176720133926No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA29590.12249118676118236No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA28700.11880693004548608No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT26990.1117281896142045No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC25500.10556016432612875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.070.996611
GTATCAA76750.059.6344381
TATCAAC100300.045.5731962
ATCAACG102150.044.747843
CAACGCA105800.043.0915685
TCAACGC106150.043.0616234
AACGCAG107050.042.913996
ACGCAGA109850.041.7659727
CGCAGAG111950.041.028878
GCAGAGT117000.039.158699
CAGAGTA118700.031.45638510-11
AGAGTAC113100.030.54115310-11
ACGGGTT5950.027.0082235
ACGCGTA450.00953605726.44209398-99
GTACGGA4900.025.5077671
GAGTACG141800.025.3885812-13
GTACTGG16750.024.8732431
AGTACGG140500.023.84467112-13
GAAAAAA88650.022.1556431
CGTTAAC1903.299812E-521.9277271
Produced by FastQC (version 0.11.5)