FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004912817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062306
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA105580.2599016420722614No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC82740.2036774186878093No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG77170.18996599468380768No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC75630.18617504442058277No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT74510.18341798968369197No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG74280.1828518088002233No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC73000.17970088910091953No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA71560.17615610443920277No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA71010.17480219363090818No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC70810.17430986242789193No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT69450.17096201024738167No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT67850.1670233606232519No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT63080.1552812614313151No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG62790.1545673811869416No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA59410.14624698385596752No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA59280.14592696857400697No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT58670.14442535840480752No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG56700.13957589605509776No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA56420.13888663237087506No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA52430.12906462487070153No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT51820.12756301470150205No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG50990.12551984020898474No Hit
CCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGG50910.12532290772777827No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG50720.12485519308491286No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC49790.12256585299088744No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA48390.11911953456977392No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC47310.11646094607348635No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT46690.11493471934413606No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG46630.1147870199832312No Hit
GTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACC46620.11476240342308039No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT46560.11461470406217553No Hit
ACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAG46250.1138515906975004No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCG44100.10855903026507603No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTC43920.10811593218236146No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG43900.10806669906205982No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC43650.10745128505828955No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT43630.10740205193798794No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA43540.10718050289663063No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT43420.1068851041748209No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG42710.10513732840411333No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC42350.10425113223868412No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC41220.10146946094164251No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAAT40860.10058326477621331No Hit
GTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC40720.10023863293410196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)