FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4273215
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190660.44617460155877947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132810.31079643781087546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96810.226550735219267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80250.076.908551
GTATCAA167050.061.9218831
TATCAAC229700.047.8233952
ATCAACG234900.046.7140583
CAACGCA242450.045.106825
TCAACGC246100.044.562884
AACGCAG249100.044.0698476
CGTATCA7300.043.2109571
CTATCAA17100.042.4624061
ACGCAGA263550.041.516177
CGCAGAG280250.039.0846948
GCAGAGT284400.038.514379
AGAGTAC278700.030.46390510-11
CAGAGTA291750.029.87619810-11
GAGTACT139950.029.0366412-13
AGTACGG172800.026.52940212-13
GAGTACG175450.026.4847712-13
GTGGTAT44000.023.6715241
GTACGGA4900.023.0780121
GTACTGG22400.021.7874641
Produced by FastQC (version 0.11.5)