FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4273215
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59960.14031589798313449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.066.569491
GTATCAA102850.060.906171
TATCAAC137100.045.5170022
CAACGCA139600.044.4032865
ATCAACG141500.043.680823
TCAACGC144500.043.02124
AACGCAG146050.042.469156
ACGCAGA149750.041.298167
CGCAGAG161100.038.4521188
GCAGAGT163300.038.0829629
AGAGTAC157450.030.75096510-11
CAGAGTA168450.029.60853210-11
GAGTACG183050.026.32123412-13
AGTACGG179450.026.25225312-13
GTACGGG218350.021.93120414-15
TACGGGC45150.021.29140914-15
GTACTGG22100.021.285541
TACGGGT21350.021.196574
GGGCTAT20200.020.6278576
ATACCGA6900.019.8420336
Produced by FastQC (version 0.11.5)