FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126949
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142870.4568990412059807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104250.33339207003376137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83570.2672573169565605No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA68980.2205984171791737No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC57310.18327769336820013No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC47580.15216110016504908No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG45540.14563716901043158No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA44170.14125590151934042No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44010.14074422064446845No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA41540.13284514713863257No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT39580.12657705642145106No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT38350.12264350969587287No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG37510.1199571851027951No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT36480.11666323947080684No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA35040.1120581115969592No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC34010.10876416596497097No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG33960.10860426569157348No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG33540.10726110339503458No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA32980.10547022033298271No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG31980.10227221486503296No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA31780.10163261377144303No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT31680.10131281322464805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.078.448541
GTATCAA167050.060.3450781
TTACGGG3650.044.0716061
ATCAACG235800.043.3732953
TATCAAC240200.042.8017042
TCAACGC244900.041.810224
CAACGCA245900.041.6159975
AACGCAG249450.041.0475966
ACGCAGA264200.038.7109157
CGCAGAG266550.038.3919458
GCAGAGT277950.036.795919
AGAGTAC276150.031.304810-11
CAGAGTA279350.029.50857410-11
GAGTACT129400.026.34575812-13
GTACTGG20700.025.6157841
AGTACGG205550.024.51637612-13
TGTATCA11750.023.8313141
GAGTACG203700.023.70215612-13
GGTTCAC27250.023.3618726
GTTCACT27750.022.7265367
Produced by FastQC (version 0.11.5)