FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126949
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA65750.210268859517696No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC57990.18545233708640596No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC50600.1618190766782573No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG46670.1492509151892148No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA45520.1455732089010726No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT44760.14314272474543077No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA43940.14052036026171197No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43720.139816799058763No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG43410.1388254173636986No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT40340.12900754057709288No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39470.12622527581997658No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA38160.12203588865696242No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT37070.11855006269689719No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT36730.11746274083779429No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG35190.11253781241715166No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG34960.11180227115952324No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA33590.10742100366843206No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC32010.10236815502907147No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA31820.10176053399016101No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG31550.10089707251381458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.069.434281
GTATCAA113550.058.4258081
ATCAACG145450.044.8755573
CAACGCA151200.043.2083325
TATCAAC152650.043.1876832
TCAACGC153300.042.655254
AACGCAG154050.042.5634546
ACGCAGA156000.041.9169857
CGCAGAG157200.041.710568
GCAGAGT165150.039.738729
AGAGTAC162650.030.91146710-11
CAGAGTA166300.030.8949210-11
TAGACCG1004.440364E-429.7500025
GTACTGG23150.028.5291581
CATACCG850.00721223628.0000023
GAGTACG203000.024.94310212-13
AGTACGG204250.024.29522912-13
TACGGGT28000.023.3749984
GGTTCAC29250.022.9863246
CGGGACG3500.022.09999816-17
Produced by FastQC (version 0.11.5)