FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963958
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76930.2595515860886018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70800.2388697815556091No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG67540.22787097522974348No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA59390.2003739594150794No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC43000.14507627975835016No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT42280.14264709553914057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.13137837985558498No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG37500.12652001141716582No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA36840.12429325921622372No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA36440.12294371242777395No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG35030.11818655999848851No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT33940.11450904499996288No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA33270.11224855412930954No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT32460.10951572188269874No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT32090.10826739110338271No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC31730.1070527989937779No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA31290.10556829752648317No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG31170.10516343348994824No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC30440.1027005106010274No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA30210.10192452119766879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.096.69781
AAGTACG1000.077.449461
ATCGTAA507.3983047E-659.576511
GTATCAA102550.058.501751
ATCAACG138800.044.752193
ACAGCGT951.4736361E-743.8984831
CAACGCA144100.043.10625
TCAACGC144850.042.924084
AACGCAG149650.041.7063336
ACGCAGA158050.039.37687
CGCAGAG159400.039.005988
AGGATCG801.19815E-437.1863257
GCAGAGT167550.037.1441579
TATCAAC170000.036.5738452
ACGTCAG1001.0534346E-535.7459071
GTCGTGC2300.033.673681
TAGCGTG1451.09910616E-732.826559
CAGAGTA175750.029.63903410-11
AGAGTAC178450.028.30703410-11
GTACTGG11250.027.0080171
Produced by FastQC (version 0.11.5)