FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813514
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.072.967661
GTATCAA89550.061.5929681
ATCAACG111700.048.3137863
TCAACGC115100.046.8349274
CAACGCA115950.046.4402775
TATCAAC117500.046.435322
AACGCAG117350.045.9876446
ACGCAGA121050.044.4345327
CGCAGAG123150.043.6768158
GCAGAGT133000.040.397379
AGAGTAC126100.033.35963410-11
CAGAGTA130800.032.297410-11
AGTACGG149400.027.95783212-13
GAGTACG152750.027.247312-13
TACGGGT19850.024.42947214-15
GTACGGG175050.023.07940714-15
ACGGGAT20100.022.34950316-17
TACGGGC28250.022.11504614-15
ACGGGAA25550.021.1917816-17
TACGGGA81350.021.02796614-15
Produced by FastQC (version 0.11.5)