FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685906
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141730.5276804177063531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79490.2959522783001341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59030.21977686486422088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.079.2051851
GTATCAA111050.063.2622031
ATCAACG139600.049.864813
TATCAAC143500.048.799832
TCAACGC143650.048.790294
CAACGCA146900.047.6703575
AACGCAG148750.047.277476
ACGCAGA160500.043.6680687
CGCAGAG165750.042.356718
GCAGAGT176750.039.7206469
TAACGCA3750.034.90484
AGAGTAC168500.032.46725510-11
CAGAGTA172300.031.47495710-11
ATAACGC4300.029.0565833
GTATAAC5750.029.0161151
GAGTACG105800.027.3865312-13
GAGTACT93150.027.0178812-13
AGTACGG101050.026.99583412-13
AGTACTT115000.023.90219912-13
GTACGGG119500.023.64936314-15
Produced by FastQC (version 0.11.5)