FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004912960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480909
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128410.5175925437007162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89450.3605533294449736No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC75780.30545255791324877No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC73850.29767315125222243No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT68710.2769549386938417No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA67250.2710699989399047No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA66050.2662330621558469No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG62370.25139978935140306No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG57170.23043972995381934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56240.2266911039461746No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA55690.22447417458681473No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG55640.2242726355541457No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT54320.2189520050916821No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT54000.21766215528260002No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52490.211575676495994No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC51290.2067387397119362No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT51270.2066581240988686No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT50810.20480396499831313No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA50540.2037156542219001No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG49280.19863687059863944No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA47500.1914620810356204No Hit
CCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGG46450.18722976134956984No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC45620.18388421340726321No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC43210.17417003203261386No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA41000.16526200678864078No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC40730.16417369601222775No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG40670.1639318491730249No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCG40310.16248076813780754No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG40210.16207769007246942No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT39110.15764383135374976No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT38000.15316966482849634No Hit
CTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCG37920.15284720237622582No Hit
TATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTG37390.15071088862993362No Hit
GGTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCT37200.14994504030579114No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG36910.14877611391631052No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA36900.1487358061097767No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT36550.14732503288109317No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA35620.1435764068734484No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC35600.14349579126038078No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG34640.13962624183313455No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA34130.13757054369991No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTC33740.13599853924509123No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC33240.1339831489184005No Hit
GTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACC33050.133217300594258No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGC32840.13237083665704788No Hit
CCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGT32790.1321692976243788No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAAT32430.13071821658916147No Hit
GTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATT32360.13043606194342477No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC32190.1297508292323499No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG32100.12938805897354558No Hit
GTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC32050.1291865199408765No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT32040.1291462121343427No Hit
CGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGC31100.1253572783201641No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC31000.12495420025482595No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC30840.12430927535028492No Hit
ACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAG30680.12366435044574389No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT30630.1234628114130748No Hit
GGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGC30250.12193111476478985No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA30200.12172957573212076No Hit
CCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAG30080.121245882053715No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA29940.12068157276224159No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA29780.12003664785770055No Hit
TCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGA29390.11846464340288176No Hit
CCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTG29350.11830341217674652No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG29340.1182631043702127No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCG29190.11765848727220546No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC29180.11761817946567166No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGG28990.11685233114152917No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATAC28850.11628802185005577No Hit
CCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT28810.1161267906239205No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG28730.11580432817164998No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGG28530.11499817204097369No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT28340.11423232371683122No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC28280.11399047687762832No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC28240.11382924565149306No Hit
GTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG27440.11060462112878787No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC27310.11008061964384828No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG26840.10818615273675898No Hit
GTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTA26810.10806522931715755No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC26660.1074606122191503No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGG26420.10649322486233877No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT25760.103832909631107No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA25390.10234152078935585No Hit
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTC25360.1022205973697544No Hit
CCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCT25340.10213998175668676No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG25300.1019787505305515No Hit
ATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGT25250.10177721149788244No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGT25220.10165628807828099No Hit
GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA25000.10076951633453705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)