FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3222739
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136100.4223115803048277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100050.31045021020939023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.2568622528848908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68400.080.050131
GTATCAA144250.059.683551
TAGTACG2650.047.2145731
TATCAAC195850.045.6898842
ATCAACG197450.045.168983
TCAACGC203950.043.758594
CAACGCA205300.043.4128845
AACGCAG213500.041.8569766
CGCAGAG225700.039.488998
ACGCAGA227050.039.25427
GCAGAGT242100.036.8631369
AGAGTAC234100.031.75635110-11
CAGAGTA243050.030.15857910-11
TTCAACG5050.029.4951951
GAGTACT121600.027.37551512-13
GAGTACG152150.026.2193912-13
TTACGGG4100.026.1572041
AGTACGG155300.025.38108812-13
CGTCAGA7300.024.4508615
GACCGTT4450.024.0662427
Produced by FastQC (version 0.11.5)