FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424134
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569830.8870144987635686No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348650.5427190653246026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316080.4920196247463082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249530.38842589522572224No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG219800.3421472839763305No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA124250.1934112831394862No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT121530.18917724941603023No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT112510.1751364464066285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109850.17099581048589585No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA108510.16890992622507564No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC98050.15262757595031487No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG97200.15130444041173488No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA90290.1405481267981023No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT84330.1312706117275885No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG78630.12239782046887565No Hit
GTACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA77680.12091902192575685No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC76390.11891096916720603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76340.11883313766493664No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG73670.11467693544375007No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA70310.10944665849124567No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC68180.10613103649456876No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT67890.10567961378140618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147750.063.1449171
GTATCAA329600.056.774491
GCTCTAG4450.052.146411
ATCAACG406500.046.147633
GTACTTT614400.045.2257161
TCAACGC418050.044.929584
CAACGCA422350.044.4862375
GAGTCGG7150.044.1051831
CGGGGAC8600.043.5841034
ACTTTTT648450.043.3705673
TCTGTCG5100.043.1598978
AACGCAG436900.043.126276
TACTTTT698650.040.254582
ACGGGAC28600.040.1492353
ACGCAGA470550.040.0042887
CGCAGAG472850.039.8449678
TATCAAC479100.039.2170872
GCAGAGT487500.038.647589
TACGCGG1401.897206E-938.247572
CCCGTTG12150.035.2567444
Produced by FastQC (version 0.11.5)