FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6425003
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570530.8879840211747762No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342300.5327623971537445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313220.48750171789803054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248540.3868325042027218No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG214640.3340698829245683No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA125040.1946146951215431No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT122840.19119057220673671No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT110280.17164194320220552No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109810.17091042603404233No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA106180.16526062322461174No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC97200.1512839760541746No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG95860.14919837391515614No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA88320.1374629708344105No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT83030.12922951164380778No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG78050.12147854250029146No Hit
GTACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA76670.11933068358100378No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76050.11836570348683105No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC75280.1171672604666488No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG72320.11256025872672744No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA70740.11010111590609374No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT68540.10667699299128733No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC65870.10252135290831771No Hit
GTACGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAAC65070.10127621730293355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152100.063.85911
GTATCAA333000.055.8697361
GCTCTAG4900.052.2281041
GCCCGTT10600.048.8398443
CAGTACG1400.046.762371
CCCGTTG11150.046.4307024
ATCAACG411350.045.264213
GTACTTT616000.045.1488721
CGGGGAC8850.045.0497554
TCAACGC419800.044.46654
CAACGCA423050.043.9694865
CGTTACG1109.635187E-943.2764325
ACTTTTT656150.042.850863
AACGCAG435400.042.7836466
ACGGGAC27000.042.3153883
GAGTCGG6550.041.7973181
ACGCAGA468350.039.773677
TACTTTT707550.039.7379572
CGCAGAG471950.039.5459188
TATCAAC479050.039.0413132
Produced by FastQC (version 0.11.5)