FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6490449
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568500.8759024221590833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323060.4977467660557845No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311520.47996679428495626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244380.37652248711915004No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG215620.3322112229831865No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA125680.19363837540361228No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT123510.19029500116247736No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT115670.17821571358160276No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA111490.17177548117241195No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106200.16362504350623508No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG99050.15260885649051398No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC96230.1482640107024953No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA92200.14205488711181616No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT85420.1316087685150904No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG80600.12418247181358333No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC79710.12281122615708097No Hit
GTACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA78940.12162486755538791No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77010.11865126742387161No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG73640.11345902263464362No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT73060.11256540186973198No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA71620.11034675721202031No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC68610.10570917358722023No Hit
GTACGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAAC67810.10447659322182488No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA65880.10150299309030855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161800.063.0221371
GCTCTAG5300.056.2561071
GTATCAA350200.056.072661
CGTTACG1300.050.341565
ATCAACG430500.045.8082473
GTACTTT638050.044.8509451
TCAACGC440550.044.7902644
CAACGCA444450.044.401735
AACGCAG457400.043.2330066
ACGGGAC27100.042.3791583
ACTTTTT678300.042.2940863
GAGTCGG7200.042.238961
GTCTCGC5450.040.483751
ACGCAGA489900.040.3163457
CGCAGAG493600.040.0985078
GCCCGTT12850.039.362273
TACTTTT730500.039.2636952
TATCAAC504750.039.1286852
GCAGAGT507150.039.0271579
CGGGGAC10300.038.7081074
Produced by FastQC (version 0.11.5)